Isolation of DNA, Fragmentation of DNA by restriction endonucleases...
Process of Recombinant DNA Technology
Recombinant DNA technology involves the following steps:
Isolation of DNA.
Fragmentation of DNA by restriction endonucleases.
Isolation of a desired DNA fragment.
Amplification of the gene of interest.
Ligation of the DNA fragment into a vector.
Insertion of recombinant DNA into the host.
Culturing the host cells on a suitable medium at a large scale.
Extraction of the desired gene product.
Downstream processing of the products as finished product, ready for marketing.