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PCR Technique - Steps
1. Denaturation (94°C): Separation of dsDNA into single strands; 2. Annealing (52°C): Attachment of two sets of primers; 3. Extension (72°C): Taq polymerase synthesizes new DNA strands using dNTPs.
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1. Denaturation (94°C): Separation of dsDNA into single strands; 2. Annealing (52°C): Attachment of two sets of primers; 3. Extension (72°C): Taq polymerase synthesizes new DNA strands using dNTPs.