Syringe

Biotechnology: Principles and Processes

EXERCISE SOLUTIONS

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Question 1. Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).

Ans:

Human insulin – Diabetes

DNase I – Treatment of cystic fibrosis.

Chronic Gonadotropin – Treatment of infertility

Human growth hormone – Dwarfism cure

Calcitonin – Treatment of rickets

TPA (tissue plasminogen activator) – Heart attack/strokes

PDGF (platelet derived growth factor) – Stimulates wound healing.

Interferon (α, β and γ) – Treatment of viral infection and cancer.

Interlinkins – Enhances activity of  immune reaction,

Reo Pro – Prevention of blood clots.

Question 2. Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

Ans:

Name of the Restriction enzyme – Bam HI.

The substrate DNA on which it acts –

rDNA.jpg

Question 3. From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?

Ans: Even though both DNA and enzymes are macromolecules, DNA is bigger in molecular size as compared to proteins. Because DNA is